Received: AugAccepted: DecemPublished: February 18, 2015Ĭopyright: © 2015 Kakumani et al. PLoS ONE 10(2):Īcademic Editor: Miguel Lopez-Ferber, Ecole des Mines d'Alès, FRANCE The newly identified miRNAs greatly enrich the repertoire of insect miRNAs and analysis of expression profiles reveal their involvement at various steps of biochemical pathways of the army worm.Ĭitation: Kakumani PK, Chinnappan M, Singh AK, Malhotra P, Mukherjee SK, Bhatnagar RK (2015) Identification and Characteristics of microRNAs from Army Worm, Spodoptera frugiperda Cell Line Sf21. Further, we identified a total of 809 potential target genes with GO terms for selected miRNAs, involved in different metabolic and signalling pathways of the insect. In addition, 12 miRNAs were validated using northern blot analysis and putative functional role assignment for 6 S f miRNAs was investigated by examining their relative abundance at different developmental stages of Spodoptera litura and body parts of 6 th instar larvae. A total of 5 miRNA clusters were identified and the largest one encodes 5 miRNA genes.

Landscape distribution analysis based on Sf21 genome assembly revealed clustering of few novel miRNAs. mori, Drosophila melanogaster and Tribolium castenum while the remaining 110 miRNAs were identified as novel based on comparative analysis with the insect miRNA data set. Of the total, 116 miRNAs were well conserved within other insects, like B. In the present study, we identified a total of 226 miRNAs from Spodoptera frugiperda cell line Sf21.

Amongst lepidopteran insects, miRNAs from only Bombyx mori have been studied extensively with a little focus on Spodoptera sp. MicroRNAs play important regulatory role in all intrinsic cellular functions.

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